Analyse of iPBS lenght polymorphism in selected group of Vitis vinifera, L varieties
Keywords:
Vitis vinifera L, iPBS, DNA markers, polymorphism, genetic distanceAbstract
Here, the specific natural variability of iPBS (Inter Primer Binding Sites Polymorphism) fingerprints in thirteen varieties of Vitis vinifera, L. was performed. All of the analysed biological material was collected in the vineyard of Sabo vinery for describing of the existing genetic polymorphism. Young leaves from a total of thirteen grapevine varieties were obtained in the in the Small Carpathians wine region of Slovakia, Vrbové. Genetic length polymorphism was studied by iPBS markers. A dendrogram of genetic similarity of generated fingerprints was constucted by UPGMA (Unweighted Pair Group Method with Arithmetic mean) and the Jaccard coefficient of genetic similarity was used for the analyse of 13 Vitis vinifera, L varieties. The generated dendrogram is separated into three major clusters at the genetic dissimilarity of 0.58. Cluster 1 is composed of two red varieties – Alibernet and Cabernet Sauvignon. Cluster 2 was further subdivided into two sub-clusters, where the larger one include all white varieties - Pinot Blanc, Müller-Thurgau, Welschriesling, Irsai Oliver, Grüner Veltliner, Pálava, Weisser Riesling, Sauvignon Blanc and Feteasca Regala. The second subcluster is comprised from two red varieties – Blaufränkisch and Dornfelder. The analysis prooved the the iPBS technique is an effective retrotransposon based markers to evaluate the variability of the genome in the germplasm of Vitis vinifera L. cultivated varieties.
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