Optimization, characterization and biological effect of Algerian propolis


  • Zahra Toutou University of BEJAIA
  • Sofiane Fatmi bejaia university https://orcid.org/0000-0001-9925-7831
  • Nacera Chibani
  • Leila Bensidhoum
  • Mohamed Skiba university of ROUEN
  • Mokrane Iguerouada University of BEJAIA


biological activities, experimental design, high liquid performance chromatography (HPLC), propolis, sperm motility.


Natural products was the source of many important biological activities against human and animal pathogens. Propolis was one of these substances, produced by bees from resins and wax. In this study, D-optimal experimental design used to optimize operational condition of propolis extraction, and then phenolic compounds of propolis extracted from seven regions of Bejaia was identified and quantified by HPLC analysis. Also, their biological activities was evaluated, including antibacterial, antifungal, antioxidant, anti-inflammatory effects, and sperm cells cryopreservation effect. The highest antibacterial activitywas obtained in propolis extract from the Baccaro region against B. cereus and strong effect against S. areus, C. albicans, and E. Coliwas reached using propolis extracted from Melbou region. High inhibition percentage denaturation of the BSA protein was observed using propolis extracted from Melbou. Also, the best antioxidant and sperm cells cryopreservation preservation was obtained using propolis extracted from Melbou. This work demonstrates that Melbou propolis extract obtained by the agitation method (MEA) showed the best and strongest biological effects, indicating an interesting and promising approach for development of new therapeutics formulations based on propolis extract from bejaia region. 


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Animal Science