Preeliminary screening of endophytic bacteria associated with rootsof potato plant grown in middle altitude as antagonist against bacterial wilt disease caused by Ralstonia solanacearum
Keywords:biological control, bacteria wilt disease, potato wilt disease, endophytic bacteria, middle altitude
Bacterial wilt disease caused by Ralstonia solanacearum is a devastating plant disease on potato plant. This study aimed to screen endophytic bacteria isolated from potato roots planted in middle latitude areas and confirm their antagonistic potential against R. solanacearum. Endophytic bacteria were isolated from healthy potato roots (Granola cultivar) grown in the middle latitude area (500–700 m.a.s.l.) in Bumiaji Sub-District, Batu City, East Java, Indonesia. In this study, 130 endophytic bacteria were isolated. As a result, eight endophytic bacterial isolates were found as potential antagonists against potato wilt diseases (R. solanacearum) i.e., E1, E5, E6, E104, E117, E120, E121, and E129. Based on in vivo test, E1 isolate reduced the bacterial wilt disease in potato by 20.74% and significantly increased plant growth (plant height and plant dry weight). The ability of the endophytic bacteria to produce the antibiosis substance against the R. solanacearum and their ability to reduce bacterial wilt disease in vivo suggests that those bacterial isolates have the potency to be developed as the candidates for biocontrol agents against bacterial wilt disease caused by R. solanacearum. However, it is necessary to identify the molecular bacteria isolated in this study to determine which species can suppress wilt disease and increase the growth of potato plants.
Adeleke, B. S., Babalola, O. O., & Glick, B. R. (2021). Plant growth-promoting root-colonizing bacterial endophytes. Rhizosphere, 20(September), 100433. https://doi.org/10.1016/j.rhisph.2021.100433
Afzal, I., Shinwari, Z. K., Sikandar, S., & Shahzad, S. (2019). Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research, 221, 36–49. https://doi.org/https://doi.org/10.1016/j.micres.2019.02.001
Amaresan, N., Velusamy, J., Kumar, K., & Thajuddin, N. (2012). Endophytic bacteria from tomato and chilli, their diversity and antagonistic potential against Ralstonia solanacearum. Archives of Phytopathology and Plant Protection, 45, 344–355. https://doi.org/10.1080/03235408.2011.587273
Bahmani, K., Hasanzadeh, N., Harighi, B., & Marefat, A. (2021). Isolation and Identification of Endophytic Bacteria from Potato tissues and their Effects as Biological Control Agents Against Bacterial Wilt. Physiological and Molecular Plant Pathology, 116, 101692. https://doi.org/10.1016/j.pmpp.2021.101692
Basumatary, B., Das, D., Choudhury, B. N., Dutta, P., & Bhattacharyya, A. (2021). Isolation and characterization of endophytic bacteria from tomato foliage and their in vitro efficacy against root-knot nematodes. Journal of Nematology, 53(1), 1–16. https://doi.org/doi:10.21307/jofnem-2021-104
Fadiji, A. E., & Babalola, O. O. (2020). Elucidating Mechanisms of Endophytes Used in Plant Protection and Other Bioactivities With Multifunctional Prospects. Frontiers in Bioengineering and Biotechnology, 8, 467. https://doi.org/10.3389/fbioe.2020.00467
Gashaw, G., Alemu, T., & Tesfaye, K. (2014). Evaluation of disease incidence and severity and yield loss of finger millet varieties and mycelial growth inhibition of Pyricularia grisea isolates using biological antagonists and fungicides in vitro condition . Journal of Applied Biosciences, 73, 5883–5901.
Ghadamgahi, F., Tarighi, S., Taheri, P., Saripella, G. V., Anzalone, A., Kalyandurg, P. B., Catara, V., Ortiz, R., & Vetukuri, R. R. (2022). Plant Growth-Promoting Activity of Pseudomonas aeruginosa FG106 and Its Ability to Act as a Biocontrol Agent against Potato, Tomato and Taro Pathogens. Biology, 11(1). https://doi.org/10.3390/biology11010140
Gutarra, L., Herrera, J., Fernandez, E., Kreuze, J., & Lindqvist-Kreuze, H. (2017). Diversity, pathogenicity, and current occurrence of bacterial wilt bacterium Ralstonia solanacearum in Peru. Frontiers in Plant Science, 8(July), 1–12. https://doi.org/10.3389/fpls.2017.01221
Haruta, S., & Kanno, N. (2015). Survivability of Microbes in Natural Environments and Their Ecological Impacts. Microbes and Environments / JSME, 30, 123–125. https://doi.org/10.1264/jsme2.ME3002rh
Hasan, N., Farzand, A., Heng, Z., Khan, I., Moosa, A., Zubair, M., Na, Y., Ying, S., & Canming, T. (2020). Antagonistic Potential of Novel Endophytic Bacillus Strains and Mediation of Plant Defense against VerticilliumWilt in Upland Cotton. Plants, 9, 1438. https://doi.org/10.3390/plants9111438
Hayward, A. C. (1991). Biology and Epidemiology of Bacterial Wilt Caused by Pseudomonas Solanacearum. Annual Review of Phytopathology, 29(1), 65–87. https://doi.org/10.1146/annurev.py.29.090191.000433
Istifadah, N., Pratama, N., Taqwim, S., & Sunarto, T. (2018). Effects of bacterial endophytes from potato roots and tubers on potato cyst nematode (Globodera rostochiensis). Biodiversitas, 19(1), 47–51. https://doi.org/10.13057/biodiv/d190108
Kandel, S. L., Joubert, P. M., & Doty, S. L. (2017). Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms, 5(4). https://doi.org/10.3390/microorganisms5040077
Karim, Z., Hossain, M., & Begum, M. (2018). Ralstonia solanacearum: A Threat to Potato Production in Bangladesh. Fundamental and Applied Agriculture, 3, 1. https://doi.org/10.5455/faa.280361
Kheirandish, Z., & Harighi, B. (2015). Evaluation of bacterial antagonists of Ralstonia solanacearum, causal agent of bacterial wilt of potato. Biological Control, 86. https://doi.org/10.1016/j.biocontrol.2015.03.007
Kurabachew, H., & Ayana, G. (2017). Bacterial Wilt caused by Ralstonia solanacearum in Ethiopia: StatusaAnd Management Approaches: A Review. International Journal of Phytopathology, 5, 107–119. https://doi.org/10.33687/phytopath.005.03.1829
Kurabachew, H., & Wydra, K. (2013). Characterization of plant growth promoting rhizobacteria and their potential as bioprotectant against tomato bacterial wilt caused by Ralstonia solanacearum. Biological Control, 67(1), 75–83. https://doi.org/https://doi.org/10.1016/j.biocontrol.2013.07.004
Lahlali, R., Ezrari, S., Radouane, N., Kenfaoui, J., Esmaeel, Q., El Hamss, H., Belabess, Z., & Barka, E. A. (2022). Biological Control of Plant Pathogens: A Global Perspective. In Microorganisms (Vol. 10, Issue 3). https://doi.org/10.3390/microorganisms10030596
Lee, J. H., Anderson, A. J., & Kim, Y. C. (2022). Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests. Microorganisms, 10(5), 1053. https://doi.org/10.3390/microorganisms10051053
Manter, D. K., Delgado, J. A., Holm, D. G., & Stong, R. A. (2010). Pyrosequencing reveals a highly diverse and cultivar-specific bacterial endophyte community in potato roots. Microbial Ecology, 60(1), 157–166. https://doi.org/10.1007/s00248-010-9658-x
Meng, F. (2013). Ralstonia Solanacearum Species Complex and Bac(1) Meng, F. Ralstonia Solanacearum Species Complex and Bacterial Wilt Disease. J. Bacteriol. Parasitol. 2013, 4. https://doi.org/10.4172/2155-9597.1000e119.terial Wilt Disease. Journal of Bacteriology & Parasitology, 04. https://doi.org/10.4172/2155-9597.1000e119
Miliute, I., Buzaite, O., Baniulis, D., & Stanys, V. (2015). Bacterial endophytes in agricultural crops and their role in stress tolerance: a review Inga. Zemdirbyste, 102(4), 465–478. https://doi.org/10.13080/z-a.2015.102.060
Morales-Cedeño, L. R., Orozco-Mosqueda, M. del C., Loeza-Lara, P. D., Parra-Cota, F. I., de los Santos-Villalobos, S., & Santoyo, G. (2021). Plant growth-promoting bacterial endophytes as biocontrol agents of pre- and post-harvest diseases: Fundamentals, methods of application and future perspectives. Microbiological Research, 242, 126612. https://doi.org/https://doi.org/10.1016/j.micres.2020.126612
Nandhini, S., Vaithiyanathan, S., & Babu, S. (2012). Endophytic bacteria from tomato and their efficacy against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen. Journal of Biopesticides, 5, 178–185.
Pageni, B. B., Lupwayi, N. Z., Akter, Z., Larney, F. J., Kawchuk, L. M., & Gan, Y. T. (2014). Plant growth-promoting and phytopathogen-antagonistic properties of bacterial endophytes from potato (Solanum tuberosum L.) cropping systems. Canadian Journal of Plant Science, 94(5), 835–844. https://doi.org/10.4141/CJPS2013-356
Patil, V., Gopal, J., & Singh, B. (2012). Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches. Agricultural Research, 1. https://doi.org/10.1007/s40003-012-0034-6
R Core Team. 2020. R: A language and environment for statistical computing. R Found Stat Comput. https://ww-w.r-project.org.
Rizzo, D., Lichtveld, M., Mazet, J., Togami, E., & Miller, S. (2021). Plant health and its effects on food safety and security in a One Health framework: four case studies. One Health Outlook, 3. https://doi.org/10.1186/s42522-021-00038-7
Scherf, J. M., Milling, A., & Allen, C. (2010). Moderate temperature fluctuations rapidly reduce the viability of Ralstonia solanacearum race 3, biovar 2, in infected geranium, tomato, and potato plants. Applied and Environmental Microbiology, 76(21), 7061–7067. https://doi.org/10.1128/AEM.01580-10
Soto, M., López-Lara, I., Geiger, O., Romero-Puertas, M., & van Dillewijn, P. (2021). Rhizobial Volatiles: Potential New Players in the Complex Interkingdom Signaling With Legumes. Frontiers in Plant Science, 12, 698912. https://doi.org/10.3389/fpls.2021.698912
Souza, A., da Cruz, J., Sousa, N., Procópio, A., & Silva, G. (2014). Endophytic bacteria from banana cultivars and their antifungal activity. Genetics and Molecular Research : GMR, 13, 8661–8670. https://doi.org/10.4238/2014.October.27.6
Swastika, D. K. S., Saptana, & Gunawan, E. (2021). The feasibility of Indonesian potato’s farming and its global competitiveness. IOP Conference Series: Earth and Environmental Science, 892(1). https://doi.org/10.1088/1755-1315/892/1/012006
Tsuchiya, K., Horita, M., Kurose, D., Suga, Y., Yano, K., Takeshita, M., Furuya, N., & Wai Wai Kyaw, H. (2012). Diversity of Bacterial Wilt Pathogen In Asia And Their Control Strategies. Food and Fertilizer Technology Center, Extension.
Vyas, P. (2021). Chapter 7 - Endophytic microbes: an array of organic volatiles and secondary metabolites. In A. Kumar, J. Singh, & J. B. T.-V. and M. of M. Samuel (Eds.), Volatiles and Metabolites of Microbes (pp. 105–141). Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-12-824523-1.00004-3
Wakimoto, S., Hirayae, K., Tsuchiya, K., Kushima, Y., Furuya, N., & Matsuyama, N. (1986). Production of antibiotics by plant pathogenic Pseudomonads. Japanese Journal of Phytopathology, 52(5), 835–842. https://doi.org/10.3186/jjphytopath.52.835
Wang, L., Hu, C., & Shao, L. (2017). The Antimicrobial Activity of Nanoparticles: Present Situation and Prospects for the Future. International Journal of Nanomedicine, 12, 1227–1249. https://doi.org/10.2147/IJN.S121956
Zaghloul, R., Abou-Aly, H., Tewfike, T., & Ashry, N. (2016). Isolation and Characterization of Endophytic Bacteria Isolated from Legumes and Non-Legumes Plants in Egypt. Journal of Pure and Applied Microbiology, 10(1), 277–290.
- 2023-07-12 (2)
- 2023-07-12 (1)
Copyright (c) 2023 Luqman Qurata Aini, Abdul Latief Abadi, Mintarto Martosudiro, Muhammad Akhid Syib‘li, Tita Widjayanti
This work is licensed under a Creative Commons Attribution 4.0 International License.