Grape pomace in equine nutrition: effect on antioxidant status

Renata Kolláthová, Branislav Gálik, Marko Halo, Anton Kováčik, Ondrej Hanušovský, Michal Rolinec, Miroslav Juráček, Milan Šimko


Received: 2021-10-04 | Accepted: 2021-11-29 | Available online: 2021-12-31

Grape pomace is a bioactive compound rich winery by-product having antioxidant properties. However, its use in equine nutrition in this regard have been unexploited to date. Thus, this study aimed to investigate whether dried grape pomace (DGP) could enhance the antioxidant mechanisms of horses. Redox status was assessed through glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity in blood serum, and ferric reducing ability of plasma (FRAP). Twelve horses were assigned to three groups recieving a basal diet (control group) or the basal diet supplemented with 200 g of DGP (experimental group 1), or 400 g of DGP (experimental group 2) for 30 days. Dietary DGP supplementation of horses at a level of 200 g positively affected their redox status through increased FRAP (P<0.05). However, no changes in the activity of enzymes GPx and SOD were detected neither at the level of 200 g nor 400 g of DGP. Based on the presented results, further research is required to test other levels of DGP in horse diets and its potential to affect the redox status of these animals.

Keywords: grape pomace, horses, SOD, GPx, FRAP


Alía, M., Horcajo, C., Bravo, L. and Goya, L. (2003). Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. Nutrition Research, 23(9), 1251–1267.

Balea, Ş. S. et al. (2018). Polyphenolic compounds, antioxidant, and cardioprotective effects of pomace extracts from Fetească Neagră Cultivar. Oxidative medicine and cellular longevity, 2018.

Benzie, I. F. and Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70–76.

Brenes, A. et al. (2008). Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poultry science, 87(2), 307–316.

Brenes, A. et al. (2016). Use of polyphenol-rich grape byproducts in monogastric nutrition. A review.  Animal Feed Science and Technology, 211, 1–17.

Buffa, G. et al. (2020). Supplementation of by‐products from grape, tomato and myrtle affects antioxidant status of dairy ewes and milk fatty acid profile. Journal of animal physiology and animal nutrition, 104(2), 493–506.

Chamorro, S. et al. (2017). Addition of exogenous enzymes to diets containing grape pomace: Effects on intestinal utilization of catechins and antioxidant status of chickens. Food Research International, 96, 226–234.

Chedea, V. S. et al. (2019). Red grape pomace rich in polyphenols diet increases the antioxidant status in key organs – kidneys, liver, and spleen of piglets. Animals, 9(4), 149.

Davies, J. A. et al. (2009). Feeding grape seed extract to horses: effects on health, intake and digestion.  Animal,  3(3), 380–384.

Ebrahimzadeh, S. K. et al. (2018). Effects of grape pomace and vitamin E on performance, antioxidant status, immune response, gut morphology and histopathological responses in broiler chickens. South African Journal of Animal Science, 48(2), 324–336.

Fan, Z. et al. (2015). Effects of catechins on litter size, reproductive performance and antioxidative status in gestating sows. Animal Nutrition, 1(4), 271–275.

Gladine, C. et al. (2007). Plant extracts rich in polyphenols (PERP) are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed n−3 PUFA supplemented diets. Animal Feed Science and Technology,  136(3–4), 281–296.

Goñí, I. et al. (2007). Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility, and susceptibility to meat lipid oxidation in chickens.  Poultry science, 86(3), 508–516.

Gungor, E., Altop, A. and Erener, G. (2021). Effect of Raw and Fermented Grape Pomace on the Growth Performance, Antioxidant Status, Intestinal Morphology, and Selected Bacterial Species in Broiler Chicks. Animals, 11(2), 364.

Hellwig, M. (2019). The chemistry of protein oxidation in food. Angewandte Chemie International Edition, 58(47), 16742– 16763.

Hao, R. et al. (2015). Effects of grape seed procyanidins on growth performance, immune function and antioxidant capacity in weaned piglets.  Livestock Science,  178, 237–242.

Hosseini-Vashan, S. J. et al. (2020). The growth performance, plasma biochemistry indices, immune system, antioxidant status, and intestinal morphology of heat-stressed broiler chickens fed grape (Vitis vinifera) pomace. Animal Feed Science and Technology, 259, 114343.

Ishida, K. et al. (2015). Effects of feeding polyphenol‐rich winery wastes on digestibility, nitrogen utilization, ruminal fermentation, antioxidant status and oxidative stress in wethers. Animal Science Journal, 86(3), 260–269.

Kafantaris, I. et al. (2017). Grape pomace improves antioxidant capacity and faecal microflora of lambs.  Journal of Animal Physiology and Animal Nutrition,  101(5), e108–e121.

Kafantaris, I. et al. (2018). Grape pomace improves performance, antioxidant status, fecal microbiota and meat quality of piglets. Animal, 12(2), 246–255.

Kalli, E. et al. (2018). Novel application and industrial exploitation of winery by-products.  Bioresources and Bioprocessing, 5(1), 1–21.

Kerasioti, E. et al. (2017). Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep. Toxicology reports, 4, 364– 372.

Kolláthová, R. et al. (2019). The determination of antioxidant activity and content of polyphenols in grape pomace. In Book of Abstracts of the 70th Annual Meeting of the European Federation of Animal Science (668). Wageningen, Wageningen Academic Publishers.

Kolláthová, R. et al. (2020). Effect of dietary grape pomace on fats digestibility in horses.  Acta Fytotechnica et Zootechnica, 23(5), 132–136.

Kovacik, A. et al. (2019). Trace metals in the freshwater fish Cyprinus carpio: Effect to serum biochemistry and oxidative status markers.  Biological trace element research,  188(2), 494– 507.

Lipinski, K. et al. (2017). Polyphenols in monogastric nutrition – A review. Annals of Animal Science, 17(1), 41.

Luchian, C. E. et al. (2019). Antioxidant and antimicrobial effects of grape pomace extracts. In BIO Web of Conferences (vol. 15, p. 04006). EDP Sciences.

Makri, S. et al. (2017). Novel feed including bioactive compounds from winery wastes improved broilers‘ redox status in blood and tissues of vital organs. Food and Chemical Toxicology, 102, 24–31. 

Mohamed Ahmed, I. A. et al. (2020). Chemical composition, bioactive compounds, mineral contents, and fatty acid composition of pomace powder of different grape varieties.  Journal of Food Processing and Preservation,  44(7), e14539.

Moldovan, M. L. et al. (2019). A Design of Experiments strategy to enhance the recovery of polyphenolic compounds from Vitis vinifera by-products through heat reflux extraction. Biomolecules, 9(10), 529.

National Research Council. (2007). National Research Council Committee nutrient requirements of horses. Niedzwiedz, А. et al. (2017). Role of free radicals in oxidative stress – Basic knowledge for clinician.  Науковий вісник ветеринарної медицини, 1, 77–81.

NRC. (2007). Nutrient requirements of horses. 6th rev. Pascariu, S. M. et al. (2017). Effects of wine by-products on growth performance, complete blood count and total antioxidant status in broilers. Brazilian Journal of Poultry Science,  19, 191– 202.

Olejar, K. J. et al. (2019). Characterization of an antioxidant and antimicrobial extract from cool climate, white grape marc. Antioxidants, 8(7), 232.

Oliveira, A. B. et al. (2017). Effects of organic vs. conventional farming systems on quality and antioxidant metabolism of passion fruit during maturation. Scientia Horticulturae, 222, 84– 89.

Sánchez‐Moreno, C., Larrauri, J. A. and Saura‐Calixto, F. (1998). A procedure to measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture, 76(2), 270–276.

Santos-Sánchez, N. F. et al. (2019). Antioxidant compounds and their antioxidant mechanism  (pp. 1–28). London, UK: IntechOpen. Singleton, V. L. and Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents.  American Journal of Enology and Viticulture,  16(3), 144–158.

Surai, P. F. (2016). Antioxidant systems in poultry biology: superoxide dismutase.  Journal of Animal Research and Nutrition, 1(1), 8.

Tvrda, E. et al. (2016). Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Animal reproduction science, 172, 10– 20.

Wang, X. et al. (2019). Effects of dietary grape seed polyphenols supplementation during late gestation and lactation on antioxidant status in serum and immunoglobulin content in colostrum of multiparous sows. Journal of animal science, 97(6), 2515–2523.

Yammine, S. et al. (2020). Characterisation of polyphenols and antioxidant potential of red and white pomace by-product extracts using subcritical water extraction.  Oeno One,  54(2), 263–278.

Yang, J. Y. et al. (2017). Effects of dietary grape proanthocyanidins on the growth performance, jejunum morphology and plasma biochemical indices of broiler chicks. Animal, 11(5), 762–770.

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