Physiological responses of genotypes soybean to simulated drought stress

Abstract

Received: 2016-11-23 | Accepted: 2016-04-14 | Available online: 2016-12-22
http://dx.doi.org/10.15414/afz.2016.19.04.157-162

The objective of this research was to investigate possible genetic variation in the sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying.  The work confirmed that the selected physiological characteristics (RWC, osmotic potential, stress index and created nodules on roots) are good evaluating parameters for the determination of water stress in plant. In the floricultural year 2014 an experiment with four genetic resources of soybean was launched. Sowing of Maverick (USA), Drina (HRV), Nigra (SVK) and Polanka (CZK) genotypes was carried out in the containers of 15 l capacity. This stress had a negative impact on the physiological parameters. By comparing the RWC values, the decrease was more significant at the end of dehydration, which was monitored in Maverick and Drina genotypes using the Nitrazon inoculants and water stress effect. Inoculated stressed Nigra and Polanka genotypes have kept higher water content till the end of dehydration period. Also the proline accumulation was monitored during the water stress, whilst higher content of free proline reached of Maverick. More remarkable decrease of osmotic potential was again registered in a foreign Drina and Maverick genotypes in the inoculated variations. Nigra and Polanka genotypes responses not so significant in the given conditions.

Key words: soybean, drought, stress, inoculation of Nitrazon

References
CANDRÁKOVÁ, E. and LÍŠKA, E. (2006) Vplyv teplotných a vlahových podmienok na tvorbu úrody hrachu siateho, Influence of Temperature and Water Conditions on Field Pea Yield Formation. Acta fytotechnica et zootechnica, vol. 9, no. 3, pp. 57-61.
DOWNIE, J. A. (1998) Functions of rhizobial nodulation genes. In Spaink, H. P., Kondorosi A. and Hooykaas, P. J. J. (ed.), Rhizobiacee. Dordrecht: Kluwer Academic Publisher, pp. 387.
FAROOQ, M. et. al. (2009) Plant drought stress: effects, mechanisms and management. Journal of Sustainable Agriculture, pp. 153–188. doi: http://dx.doi.org/10.1080/10440040802548555
FISCHER, R.A. and MAURER, R. (1978) Drought resistance in spring wheat cultivars. I. Grain yield response. Aust. J. Agric. Res., vol. 29, pp. 897–907. doi: http://dx.doi.org/10.1071/AR9780897
FREDERICK, J.R., CAMP, C.R. and BAUER, P.J. (2001) Drought-stress effects on branch and mainstem seed yield and yield components of determinate soybean. Crop Science, vol. 41, no. 3, pp. 759-763. doi: http://dx.doi.org/10.2135/cropsci2001
JAMES, A. T., LAWN R. J. and COOPER, M. (2008) Genotypic variation for drought stress response traits in soybean. I. Variation in soybean and wild Glycine spp. for epidermal conductance, osmotic potential, and relative water content. Australian Journal of Agricultural Research, vol. 59, no.7, pp. 656–669. doi: http://dx.doi.org/10.1071/AR07159
KU, Y. S., AU-YEUNG, W. K. and YUNG, Y. L. (2013) Drought Stress and Tolerance in Soybean. Chapter 10. http://dx.doi.org/ 10.5772/52945
LEPORT, L. et aL. (2003) Physiological responses of cool-season grain legumes to drought in the lowrainfall Mediterranean environment of south-western Australia. In: N.P. Saxena (Ed.), Management of Agricultural Drought: Agronomic and Genetic Options, pp. 163–172, Enfield: Science Publishers.
MALHOTRA, R.S., SARKER, A. and SAXENA, M.C. (2004) Drought tolerance in chickpea and lentil-present status and future strategies. Challenges and Strategies for Dryland Agriculture, no. 32, pp. 257–273.
MIRABELLLA, R., (2004) Role of ROP GTPases and NodFactor Signaling in Medicago Root Nodule Infection: Thesis. Wageningen: Wageningen University, 160p.
PATTERSON, T.G. and LARNE, T.A. (1983) Nitrogen fixation by soybeans. Seasonal and cultivar effects, and comparison of estimates. Crop Science, vol. 23, no.3, pp. 488-492. doi: http://dx.doi.org/10.2135/cropsci1983.0011183X002300030012x
RASAEI, B., GHOBADI, M.E. and AMIRI, M. K. (2013) Effect of osmotic potential on germination and seedling characteristics of soybean seeds. International Journal of Agriculture and Crop Sciences. vol., 5, no.11, 1265-1268.
RICCARDI, L.,POLIGNANO, G.B. and DE GIOVANNI, C. (2001) Genotypic response of Faba bean to water stress. Euphytica, vol.118, no.1. pp. 39–46. doi: http://dx.doi.org/10.1023/A:1004078017159
SANCHOLI, G., MOBASSER, H. R. and FANAEI, H. R. (2015) Effect Inoculation of Soybean Cultivars with bacteria Rhizobium japonicum in Sistan. Biological Forum – An International Journal no.1, pp. 552-558. ISSN 0975-1130.
SADEGHIPOUR, O. and ABBASI, S. (2012) Soybean response to drought and seed inoculation. World Applied Sciences Journal, vol.1, no.1, pp. 55-60. doi: http://dx.doi.org/10.1111/j.1467-6486.2004.00462.x
VIRGINIA, S., PAGAN, M. and COOPER, M. (2012) Genetic Analysis of Relative Water Content (RWC) in Two Recombinant Inbred Line Populations of Soybean [Glycine max (L.) Merr.] Journal of Plant Genome Sciences, vol. 1, no. 2. doi: http://dx.doi.org/10.5147/jpgs.2012.0058