Comparative analysis of different methods of Hedera helix DNA extraction and molecular evidence of the functionality in PCR

Abstract

Received: 2016-06-25 | Accepted: 2016-07-29 | Available online: 2016-12-22
http://dx.doi.org/10.15414/afz.2016.19.04.144-149

The most suitable method of total DNA extraction still remains the crucial step for many plant species, although there are many
different protocols and commercial kits for DNA isolation. In this study, five different extraction protocols were analysed to find out
the most appropriate method for DNA extraction from Hedera helix L. This species has numerous medical and pharmaceutical uses
and is also characterized by antioxidant effects on human body. In spite of its wide medical utilization, it belongs to those plant
species, where the genomic information is very limited. Comparing of different protocols resulted in the yield of extracted DNA
that has ranged from 6.3 to 487 ng μl-1. The purity of extracted DNA has ranged from 1.4 up to 2.0 A260/A280. All the extraction
methods used in this study were evaluated not only in term of quantity and purity of DNA but also its functionality in the restriction
endonuclease digestion and polymerase chain reaction based downstream analysis was performed.

Keywords: DNA extraction, CTAB, SDS,commercial kit, Hedera helix L.

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