Associations between LEP G2548A polymorphisms and lipids metabolism

Anna Trakovická, Nina Moravčíková, Kristína Candráková, Radovan Kasarda


The aim of this study was to determine the population structure based on identification of G2548A polymorphism in leptin gene and analyse its impact on selected traits related with lipids metabolism as well as obesity. Totally, 236 human samples were included in SNP genotyping using PCR-RFLP method and endonuclease HhaI. The impact of locus was assessed for biochemical parameters: BMI, level of total, HDL and LDL cholesterol. In population the prevalence of A allele was detected. The highest proportion was found for heterozygous individuals (0.44). The observed heterozygosity indicated relative sufficient proportion of heterozygotes but the FIS index showed positive value (0.1) that can signalize the increase of homozygotes proportion. The association analysis using GLM procedure proved significant impact of LEP genotypes on each of selected parameter. The results showed the significant role of leptin in human nutritional status and confirmed its value as candidate gene of lipids metabolism disorders.

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