Variability of DNA based amplicon profiles generated by Bet v 1 homologous among different vegetable species

Lucia Zamieskova, Jana Žiarovská


Received: 2020-10-07 Accepted: 2021-02-08 Available online: 2021-02-28

Pathogen-related 10 is a group of proteins derived from various plant species that contains homologues of Bet v 1, major
birch-pollen allergen, which are able to cross-react with an IgEs of sensitive patients and invoke allergic reactions. Despite
new sequencing technologies and the amount of data generated daily, PR-10 proteins have not been discovered in every
plant species. To describe genomic variability by the -omics sciences, it is necessary to screen an undiscovered members of the
group, map ypr-10 genes in genome and determine sequence variabilities. In this study Bet v 1 based amplified profile (BBAP)
method was used to analyse the variability of 14 vegetable species (Brassica oleracea in 4 varieties) and to compare generated
polymorphism among them. For all of the analysed species, a total number of generated amplicons was 162. The most different
profiles were generated by Pastinaca sativa and Capsicum annuum, the highest similarity was between Apium graveolens and
B. oleracea var. capitata. New type of genic molecular markers was applied successfully and proved to be an effective technique
to map variability of PR-10 group.

Keywords: PR-10, Bet v 1 homologue, vegetable, DNA marker, cross-reaction, allergy, genic molecular marker

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