The impact of the humic acid and phytobiotics on performance and carcass parameters of broiler chickens

Henrieta Arpášová, Veronika Pistová, Cyril Hrnčár, Martin Fik, Peter Haščík, Miroslava Kačániová, Branislav Gálik, Ondrej Bučko


Received: 2018-05-29 | Accepted: 2018-05-29 | Available online: 2018-11-26

The aim of this experiment was to determine the influence of humic substances, and combination humic substances and selected phytobiotics on production and carcass parameters of broiler chickens. In experiment from total 200 one-day-old ROSS 308 chickens were randomized into four groups (n = 50). The control group was fed with basal diet (BD) without any additives. Group of chickens marked as treatment 1 (T1) was fed a BD containing 2% of humic acid, the group marked as treatment 2 (T2) was fed a BD containing 78% of humic acids, 18% of garlic powder (Allium sativum L.), 1% of milled dried leaves of wormwood (Artemisia absinthium), 1% of milled dried leaves of thyme (Thymus vulgaris), 1% of milled dried leaves of oregano (Origanum vulgare) and 1% of milled dried leaves of bogbean (Menyanthes trifoliata), together 2 kg /100kg complete feed mixture (BD). In the group marked as treatment T3 were chicken fed with BD containing industrially produced coccidiostats. Experiment lasted 42 days. At the end of the experiment was average body weight (values in the order of the groups: 1808.03 ±212.39; 1981.75 ±203.32; 1895.59 ±178.75 and 1955.31 ±237.16 g ±SD) significantly higher (P<0.05) in all treatment in compare to control group. In T2 was thigh part (29.27 ±1.50; 29.07 ±3.35; 30.45 ±2.15 and 29.49 ±2.34 mean ±SD) significantly higher (P<0.05) compared to control group. Carcass weight (values in the order of the groups: 1357.18 ±95.8; 1486.38 ±156.7; 1369.69 ±118.0 and 1440.68 ±132.1 g ±SD) and carcass yield (74.35 ±1.33; 76.10 ±1.97; 74.03 ±1.35 and 73.45 ±1.82 mean ±SD) were the highest in treatment T1 with humic acid addition (P>0.05).

Keywords: Allium sativum L., Artemisa absinthium, broiler chicken, carcass parameters, humic acid, Menyanthes trifoliata, Origanum vulgare, performance parameters, Thymus vulgaris


Alipour, F., Hassanabadi, A., Golian, A., Nassiri-Moghaddam, H. (2015) Effect of plant extracts derived from thyme on male broiler performance. Poutry Science, 94 (11), 2630-2634. DOI:

Bacler-Żbikowska, B. (2012) Zasoby gatunkowe cennych roślin leczniczych powiatu włoszczowskiego. Część 3 – bobrek trójlistkowy Menyanthes trifoliata L. Annales Academiae Medicae Silesiensis, 66 (6), 7-12.

Bertella, A,  Benlahcen, K,  Abouamama, S., Pinto, D.C.G.A., Maamar, K., Kihal, M., Silva, A.M.S. (2018)  Artemisia herba-alba Asso. essential oil antibacterial activity and acute toxicity. Industrial Crops and Products, 116, 137-143.  DOI:

Ceylan, N., Çiftçi, İ., İlhan, Z. (2003) The effects of some alternative feed additives for antibiotic growth promoters on the performance and gut microflora of broiler chicks. Turkish Journal of Veterinary and Animal Sciences, 27 (3), 727-733.

Diaz-Sanchez, S., D’Souza, D., Biswas, D., Hanning, I. (2015) Botanical alternatives to antibiotics for use in organic poultry production. Poultry Science,  94 (6), 1419-1430.  DOI:

Giannenas, I., Bonos, E., Christaki, E., Florou-Paneri, P. (2018) Oregano: A Feed Additive with Functional Properties. In: Therapeutic Foods, A volume in Handbook of Food Bioengineering, 179–208.

Hafeez, A., Manner, K., Schieder, C., Zentek, J.  (2016) Effect of supplementation of phytogenic feed additives (powdered vs.encapsulated) on performance and nutrient digestibility in broiler chickens. Poultry Science,  95 (3), 622-629. DOI:

Haselmeyer, A., Zentek, J., Chizzola, R. (2014) Effects of thyme as a feed additive in broiler chickens on thymol in gut contents, blood plasma, liver and muscle. Journal of the Science of Food and Agriculture, 95 (3), 504508. DOI:


Hassan, H.M.A., M.A. Mohamed, A.W. Youssef, E.R. Hassan, (2010) Effect of using organic acids to substitute antibiotic growth promoters on performance and intestinal microflora of broilers. Asian-Australasian Journal of Animal Sciences, 23, (10) 1348-1353. DOI:

Issa, K. M., Omar, J. M. A. (2012) Effect of garlic powder on performance and lipid profile of broilers. Open Journal of Animal Sciences, 2 (2), 62-68. DOI:

Kamali Sangani A, Masoudi A.A, Hosseini S.A (2014) The effects of herbal plants on mucin 2 gene expression and performance in ascetic broilers. IJVM 8 (1), 47-52.

Khan, U, Nikousefat, Z, Tufarelli, V, Naz, S,Javdani, M, Laudadio, V. (2010) Garlic (Allium sativum L.) supplementation in poultry diets: effect on production and physiology. World's Poultry Science Journal,   68 (3), 417-24.

Kuldeep Dhama, Ruchi Tiwari, Rifat Ullah Khan et al. (2014) Growth Promoters and Novel Feed Additives Improving Poultry Production and Health, Bioactive Principles and Beneficial Applications: The Trends and Advances-A Review. International Journal of Pharmacology, 10 (3), 129-159. DOI:

Lala, A. O., Okwelum, N., Oso, A. O., Ajao, A. O., Adegbenjo, A. A. (2017) Response of Broiler Chickens to Varying Dosage of Humic Acid in Drinking Water. Journal of Animal Production Research, 29 (1), 288-294.  

Mansoub, N.H. (2011) Comparative Effects of Using Garlic as Probiotic on Performance and Serum Composition of Broiler Chickens. Annals of Biological Research, 2 (3), 486-490.

Marcinčáková, D., Mačanga, J., Nagy, J., Marcinčák, S., Popelka, P., Vašková, J., Jaďuttová, I., Mellen, M. (2015) Effect of supplementation of the diet with humic acids on growth performance and carcass yield of broilers. Folia Veterinaria, 59 (3), 165-168.

Milošević, N., Vidica Stanaćev, V.,  Perić, L., Stojčić, M. D., Veljić, M. (2013)  Effects of different levels of garlic powder in the diet on production parameters and slaughter traits of broiler chickens. Einfluss verschiedener Zulagen an Knoblauchpulver zum Futter auf Leistung und Schlachtkörpermerkmale von Broilern.  Archiv für Geflügelkunde., 77 (4), 254-259.

Nagaraju, R., Reddy, B.S., Gloridoss, R., Suresh, B.N., Ramesh, C. (2014) Effect of dietary supplementation of humic acids on performance of broilers. Indian Journal of Animal Sciences, 84 (4), 447-452.

Ozturk, E., Ocak, N., Turan, A.,  Cankaya, S. (2012) Performance, carcass, gastrointestinal tract and meat quality traits, and selected blood parameters of broilers fed diets supplemented with humic substances. Journal of the Science of Food and Agriculture, 92 (1), 59-65.

Peek, H., W.,  Landman, W., J., M.  (2011)  Coccidiosis in poultry: anticoccidial products, vaccines and other prevention strategies, 31 (3), 143-161. DOI:

Pourmahmoud, B.,  Aghazadeh, A. M., Sis, N. M. (2013) The effect of thyme extract on growth performance, digestive organ weights and serum lipoproteins of broilers fed wheat- based diets. Italian Journal of Animal Science, 12 (3), 337-341. DOI:

Ramiah, S. K., Zulkifli, I., Rahim, N. A. A., Ebrahimi, M., Meng, G. Y. (2014) Effects of Two Herbal Extracts and Virginiamycin Supplementation on Growth Performance, Intestinal Microflora Population and Fatty Acid Composition in Broiler Chickens. Asian-Australasian Journal of Animal Sciences, 27 (3), 375-382. DOI:

Samanthi, K.A.M.,  Nayananjalie, W.A.D., Adikari, A.M.J.B., Liyanag, R.  (2015) Dietary Garlic (Allium sativum L.) Supplementation on Performance, Meat Quality and Lipid Profile in Broilers. Rajarata University Journal, 3, 17-24

SAS. User’s Guide 2005. Version 9.1(TS1M3). 2005. SAS Institute Inc., Carry.

Seddiek, S.A., Ali, M. M., Khater, H. F., El-Shorbagy, M. M.  (2011) Anthelmintic activity of the white wormwood, Artemisia herba-alba against Heterakis gallinarum infecting turkey poults. Journal of Medicinal Plants Research, 5 (16), 3946-3957.

Slyranda Baltini Aji, Kennedy Ignatius, Asha´Adatu Y. Ado, Joel Bakari Nuhu, Auwal Abdulkarim, Usman Aliyu, Muhammad Bello Gambo, Mohammed Adamu Ibrahim, Haruna Akubakar, Mohammed M. Bukar, Hama´Adama M., Imam and Patrik T. Numan. (2011) Effect of feeding Onion (Allium cepa) and Garlic (Allium sativum) on some Performance Characteristic of Broiler Chickens. Research Journal of Poultry Science, 4 (2), 22-27. 

Stanaćev, V., Glamočić, D., Milošević, N., Puvača, N., Stanaćev, V., Plavša, N. (2011) Effect of garlic (Allium sativum L.) in fattening chick’s nutrition. African Journal of Agricultural Research, 6 (4), 943-948.

Šamudovská, A., Demeterová, M.  (2010) Effect of Diet Supplemented with Natural Humic Compounds and Sodium Humate on Performance and Selected Metabolic Variables in Broiler Chickens. Acta Veterinaria Brno, 79 (3), 385-393. DOI:

Taklimi, S.M.S.M., Ghahri, H., Isakan, M.A. (2012) Influence of different levels of humic acid and esterified glucomannan on growth performance  and intestinal morphology of broiler chickens. Agricultural Sciences, 3 (5), 663-668. DOI:

Toghyani, M., Tohidi, M., Gheisari, A. A, Tabeidian, S. A. (2010) Performance, immunity, serum biochemical and hematological parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. African Journal Biotechnology, 9 (40), 6819-6825.

Wunderlich, F., Al-Quraishy, S.,  Steinbrenner, H.,  Sies, H.,  Dkhil, M. A. (2014)Towards identifying novel anti-Eimeria agents: trace elements, vitamins, and plant-based natural products.  Parasitology Research, 113 (10), 3547-3556. DOI:

Zamora, G., M., Melendez, L. A. D., Hume, M.E., Vazquez, R. S.  (2017) Performance, blood parameters, and carcass yield of broiler chickens supplemented with Mexican oregano oil. Revista Brasileira de Zootecnia-Brazilian Journal of Animal Science, 46 (6), 515-520.  DOI:

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