Natural variability of restriction profiles in non-coding part of Prunus persica (L.) Batsch. Pru p 3 gene

Abstract

Article Details: Received: 2019-08-31      |      Accepted: 2019-12-29      |      Available online: 2020-03-31

https://doi.org/10.15414/afz.2020.23.01.1-6

Peach is a popular sweet fruit with a very good climate adaptation and high production. It offers many health benefits determined by its biochemical composition. However, sensitive people can be sensitised by Pru p 3 non-specific lipid transfer protein family directly or through cross reaction as a member of Bet v 1 homologues. The majority of research is focused on the protein, less data can be found in genomics or transcriptomics. This study performed RFLP analysis by chosen restricted enzymes (BfaI, MseI, NlaIII) for non-coding region of Pru p 3 (NCBI: KC311811.1) as a tool to distinguish closely related isoforms of the allergen. BfaI cut amplicon into 5 fragments corresponding to the Yulu variety in silico cleavage and polymorphism was not detected. For MseI and NlaIII polymorphisms were found in the cleavage sites, two types of restriction profiles were created for both. None of the NlaIII profiles corresponds to the restriction profile of in silico cleavage. The study confirms the varietal differences in Pru p 3 gene and supports a hypothesis that allergenicity depends on both qualitative and quantitative factors that are different and specific to each variety.

Keywords: Pru p 3, nLTP, non-coding region, restriction variability, peach

References:

AHRAZEM, O. et al. (2007) Assessing allergen levels in peach and nectarine cultivars. Annals of Allergy Asthma and Immunology, 99(1), 42–47.

ALSHAMMARY, S.F. and AL-HORAYESS, O.S. (2013) Appraisal of mineral and heavy metal contents in peach and grape grown at some major agricultural companies in Saudi Arabia. Oriental Journal of Chemistry, 29(4), 1515.

ARANZANA, M.J. et al. (2019) Prunus genetics and applications after de novo genome sequencing: achievements and prospects. Horticulture research, 6 (58). https://doi.org/10.1038/s41438019-0140-8

ARANZANA, M.J. et al. (2010) Genetic variation, population structure and linkage disequilibrium in peach commercial varieties. BMC Genetics, 11(1), 69.

BAIRD, W.V. et al. (1996) Progress in Prunus mapping and application of molecular markers to germplasm improvement. Horticulture Science, 31(7), 748–749.

BOLHAAR, S. et al. (2005) In vivo assessment with prickto-prick testing and double-blind, placebo controlled food challenge of allergenicity of apple cultivars. Journal of Allergy and Clinical Immunology, 116(5), 1080–1086.

BORGES, J.P. et al. (2006) The lipid transfer proteins (LTP) essentially concentrate in the skin of Rosaceae fruits as cell surface exposed allergens. Plant Physiology and Biochemistry, 44(10), 535–542.

BOTTON A., et al. (2002) Differential expression of two lipid transfer protein genes in reproductive organs of peach (Prunus persica L. Batsch). Plant Science, 163, 993–1000.

BOTTON, A. et al. (2009) Peach (Prunus persica L. Batsch) allergen-encoding genes are developmentally regulated and affected by fruit load and light radiation. Journal of Agricultural and Food Chemistry, 57(2), 724–734.

BOUHADIDA, M. et al. (2010) Genetic variability of introduced and local Spanish peach cultivars determined by SSR markers. Tree Genetics and Genomes, 7(2), 257–270.

BRENNA, O.V. et al. (2004) Presence of Allergenic Proteins in Different Peach (Prunus persica) Cultivars and Dependence of Their Content on Fruit Ripening. Journal of Agriculture and Food Chemistry, 52(26), 7997–8000.

CARNéS, J. et al. (2002) Pru p 3 (LTP) content in peach extracts. Allergy, 57(11), 1071–1075.

CHAE, K. et al. (2009) A gain of function mutation of Arabidopsis lipid transfer protein 5 disturbs pollen tube tip growth and fertilization. Plant Cell, 21(12), 3902–3914.

CHEN, L. et al. (2008) Genomic characterization of putative allergen genes in peach/almond and their synteny with apple. BMC Genomics, 9 (1), 543.

CHENG, Z. and HUANG, H. (2009) SSR fingerprinting Chinese peach cultivars and landraces (Prunus persica) and analysis of their genetic relationships. Science Horticulture, 120(2), 188–193.

CUBELLS-BAEZA, N. et al. (2017) Identification of the ligand of Pru p 3, a peach LTP. Plant Molecular Biology, 94(1–2), 33–44.

DUFFORT, O.A. et al. (2002) Immunoassay to quantify the major peach allergen Pru p 3 in foodstuffs. Differential allergen release and stability under physiological conditions. Journal of Agriculture and Food Chemistry, 50(26), 7738–7741.

FERNáNDEZ-RIVAS, M. et al. (2003) Clinically relevant peach allergy is related to peach lipid transfer protein, Pru p 3, in the Spanish population. Journal of Allergy and Clinical Immunology, 112(4), 789–795.

FERNáNDEZ-RIVAS, M. et al. (2006) Apple allergy across Europe: how allergen sensitization profiles determine the clinical expression of allergies to plant foods. Journal of Allergy and Clinical Immunology, 118(2), 481–488.

GAMBOA, P.M. et al. (2007) Two different profiles of peach allergy in the north of Spain. Allergy, 62(4), 408–414.

GAO, Z.S. et al. (2008). Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity. BMC Plant Biology, 13(8), 116.

GARCíA-CASADO, G. et al. (2003). Identification of IgEbinding epitopes of the major peach allergen Pru p 3. Journal of Allergy and Clinical Immunology, 112(3), 599–605.

HELSPER, J. et al. (2002). Quadrupole time-of-flight mass spectrometry: a method to study the actual expression of allergen isoforms identified by PCR cloning. Journal of Allergy and Clinical Immunology, 110(1), 131–138.

HOFFMANN‐SOMMERGRUBER, K. and SAFE consortium (2005). The SAFE project: ‘plant food allergies: field to table strategies for reducing their incidence in Europe’ an EC‐funded study. Allergy, 60(4), 436–442.

MITIC, S. et al. (2019). Comparative Study on Minerals in Peel and Pulp of Peach (Prunus persica L.) Fruit. Revista de Chimie Bucharest Original Edition, 7(6), 2281–2285.

MONACI, L. and VISCONTI, A. (2009). Mass spectrometrybased proteomics methods for analysis of food allergens Tr. In TrAC Trends. Analytical Chemistry, 28(5), 581–591.

PASQUATO, N. et al. (2006). Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein panallergens. Journal of Molecular Biology, 356(3), 684–694.

PASTORELLO, E.A, et al. (1999). The major allergen of peach (Prunus persica) is a lipid transfer protein. Journal of Allergy and Clinical Immunology, 103(3), 520–526.

REUTER, A. et al. (2006). A critical assessment of allergen component-based in vitro diagnosis in cherry allergy across Europe. Clinical and Experimental Allergy, 36(6), 815-823.

SALCEDO, G. et al. (2007). Plant non-specific lipid transfer proteins: an interface between plant defence and human allergy. Biochimistry Biophysical Acta, 1771(6), 781-791.

SALCEDO, G. et al. (2008). Review. Plant food allergens: peach non-specific lipid transfer protein Pru p 3 as a model. Spanish Journal of Agricultural Research, 6(Special issue), 30–37.

SANCHO, A.I. et al. (2008) Measurement of lipid transfer protein in 88 apple cultivars. International Archives of Allergy and Immunology, 146(1), 19–26.

VERDE, I. et al. (2013) The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nature Genetics, 45pr(5), 487–494.

WILLS, R. B. H. et al. (1983) Nutrient composition of stone fruit (Prunus spp.) cultivars: Apricot, cherry, nectarine, peach and plum. Journal of the Science of Food and Agriculture, 34(12), 1383–1389.

WüNSCH, A. et al. (2005) Molecular characterization of local Spanish peach [Prunus persica (L.) Batsch] germplasm. Genetic Resources and Crop Evolution, 53(5), 925–932.

XIE, R. et al. (2010) Evaluation of the genetic diversity of Asian peach accessions using a selected set of SSR markers. Science Horticulture, 125(4), 622–629.

YANG, Z., et al. (2011) Differential transcript abundance and genotypic variation of four putative allergen-encoding gene families in melting peach. Tree Genetics & Genomes, 7(5), 903.

YING-TAO, M. et al. (2014) The PpLTP1 Primary Allergen Gene is Highly Conserved in Peach and Has Small Variations in Other Prunus Species. Plant Molecular Biology Reporter, 32(3), 652–663.

ZHANG, A.M. et al. (2006) Genetic diversity and ecogeographical phylogenetic relationships among peach and nectarine cultivars based on simple sequence repeat (SSR) markers. Journal of American Society of Horticulture Science, 131(4), 513–521.

ZHONG-SHAN, G. et al. (2016) Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA. Food Analytical Methods, 9(4), 823–830.

ZUIDMEER, L. and van REE, R. (2007) Lipid transfer protein allergy: primary food allergy or pollen/food syndrome in same cases. Current Opinion of Allergy and Clinical Immunology, 7(3), 269–273.