Wild sunflower and goat weed leaf meals composite-mix supplementation in broiler chickens: effects on performance, health status and meat

Samuel Adebowale Adeyeye, Olugbenga David Oloruntola, Simeon Olugbenga Ayodele, Andrew Bamidele Falowo, Johnson Oluwasola Agbede


Article Details: Received: 2020-04-02 | Accepted: 2020-06-19 | Available online: 2020-12-31


Three hundred 1-day old Cobb 500 broiler chickens were randomly assigned to five experimental diets (60 birds/diet; 10 birds/ replicate) using a completely randomized design to assess the effects of wild sunflower and goat weed leaf meals composite mix (CLM) in broiler chickens. At the starter and finisher phases, a basic diet was formulated, divided into five equal parts and tagged diets 1 to 5. Diets 1 and 2 had 0% and 1.1% Oxytetracycline (Oxyt) supplementation; while the diets 3, 4 and 5 were supplemented with 0.4%, 0.8% and 1.2% CLM, respectively. In starter and finisher phases, the highest (P <0.05) body weight gain (BWG) was recorded in the birds fed diet 5 and 4, respectively compared to other diets. During the overall phase, birds fed diet 5 had the highest BWG, which was similar to those fed diet 4 but higher (P <0.05) than the birds fed the rest diets. The feed intake (FI) and feed conversion ratio (FCR) were influenced (P <0.05) by the CLM supplementation at the starter phase. The FCR recorded in birds fed the 1.1% Oxyt, 0.8% and 1.2% composite leaf mix supplemented diets (diets 2, 4, and 5) were similar (P >0.05) to those fed 0.4% CLM, but significantly better (P <0.05) the birds fed the control diet. The dietary CLM supplementation caused increased (P <0.05) serum catalase and glutathione peroxidase concentration. The meat cholesterol levels of the birds were significantly (P <0.05) reduced by dietary CLM supplementation. Conclusively, the CLM supplementation at 0.8% and 1.2% enhanced the BWG. CLM supplementation at 0.4%, 0.8 and 1.2% increased the serum glutathione peroxidase and catalase activity and reduced the broiler’s meat cholesterol.

Keywords: phytogens, avian, performance, health status, growth promoters


ADETUYI, F.O., KARIGIDI, K.O., AKINTIMEHIN, E.S. and ADEYEMO, O.N. (2018) Antioxidant properties of Ageratum conyzoides L. Asteraceae leaves. Bangladesh Journal of Scientific and Industrial Research, 53(4), 265–276.

AEBI, H. (1974) Catalase estimation. In: Bergmeyer HV (ed) Methods of enzymatic analysis. Verlag Chemic, New York Academic Press, New York.

AGIDA, G.O., FORCADOS, G.E., USMAN Y., MURAINA, I., OTTOR, M., SAMUEL, A.L., USMAN, A.S., MAKOSHI, M.S., ATIKU, A.A., GOTEP, J.G., EKUNDAYO, G.N., OLADIPO, O.O., MANCHA, M.D., OZELE, N., DIUGWU, J., MAKAMA, S., AKPOJOSEVBE, J., TONDO, B. and ELISHA, I.L. (2017). Proximate, antinutritional and mineral composition of some plants growing in Vom, Nigeria. Researchjournali’s Journal of Agriculture, 4(8), 1–8.

AOAC. (1995). Association of official analytical chemistry, official methods of analysis (16th ed.). Washington, DC: Author

BAMISHAIYE, E.I., OLAYEMI, F.F., AWAGU, E.F. and BAMISHAIYE, O.M. (2011). Proximate and Phytochemical Composition of Moringa oleifera Leaves at Three Stages of Maturation. Advance Journal of Food Science and Technology, 3(4), 233–237.

BOHM, B.A. and KOCIPAI‐ABYAZAN, C. (1994). Flavonoids and condensed tannin from leaves of Hawaiian Vaccinum vaticulatum and V. calycinium. Pacific Science, 48, 458–463.

BRUNNER, J.H. (1984). Direct spectrophotometer determination of saponin. Animal Chemistry, 34,1324–1326.

DELLES, R.M., XIONG, Y.L., TRUE, A.D., AO, T. and DAWSON, K.A. (2014). Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity. Poultry Science, 93:1561–1570.

FASUYI, A.O., DAIRO, F.A.S. and IBITAYO, F.J. (2010). Ensiling wild sunflower (Tithonia diversifolia) leaves with sugar cane molasses. Livestock Research for Rural Development, 22, 3. http://www.lrrd.org/lrrd22/3/fasu22042.htm

GLAMOCLIJ, N., SEVIC, K., BALTIC, B., BOSKOVIC, M., JANJIC, J., DJORDJEVIC, V. and MARKOVIC, R. (2016). Effects of phytobiotics on Cobb broiler production results, meatiness, and chemical composition. Meat Technology,57(2),89–94.

GUTIERREZ, R.M., BALLADA, K. and PATACSIL, M. (2015). The antibacterial property of Tithonia diversifolia (Wild Sunflower) extract from Baguio-Benguet areas in the Philippines, in response to exposure to vehicular traffic. Asian Journal of Microbiology, Biotechnology, and Environmental Sciences, 17(1), 43–52.

GYAMFI, M.A., YONAMINE, M. and ANIYA, Y. (1999). Free radical scavenging action of medicinal herbs from Ghana: thonningia sanguine on experimentally induced liver injuries. General Pharmacology, 32,661–667.

HASHEMI, S.R. and DAVOODI, H. (2010). Phytogenics as new class of feed additive in poultry industry. Journal of Animal and Veterinary Advances, 9, 2295–2304.

HUSSAIN, J., KHAN, A.L., REHMAN, N., HAMAYUN, M., SHAN, T., NISAR, M., BANO, T., SHINWARI, Z.K. and LEE, I. (2009). Proximate and nutrient analysis of selected vegetable species: A case study of Karak region, Pakistan. African Journal of Biotechnology, 8,12, 2725–2729.

IGNAT, I., VOLF, I. and POPA, I.V. (2013). Analytical Methods of Phenolic Compounds. In: Ramawat K., Mérillon JM, editors. 2061–2092. Natural Products. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6

KOSTADINOVIĆ, L., LEVIC, J., POPOVIC, S., CABARKAPA, I., PUVACA N., DURAGIC, O. and KORMAJOS, S. (2015). Dietary inclusion of Artemisia absinthium for management of growth performance, antioxidative status and quality of chicken meat. European Poultry Science, 79.  https://doi.org/10.1399/eps.2015.75

LORDING, P.M and FRIEND, S.C.E. (1991). Interpretation of laboratory results. Australian Veterinary Practitioner, 21,188–193.

MARCINČÁK, S., POPEKLA, P., ZDOLEC, N., MARTONOVA, M., SIMKOVA, J. and MARCINCAKOVA, D. (2011) Effect of supplementation of phytogenic feed Additives on performance parameters and meat Quality of broiler chickens. Slovenian Veterinary Research, 48(1), 27–34.

MISRA, H.P. and FRIDOVICH, I. (1972) The univalent reduction of oxygen by flavins and quinines. The Journal of Biological Chemistry. vol. 247, pp.188–192.

MOHITI-ASLI, M. and GHANAATPARAST-RASHTI, M. (2017) Comparison of the effect of two phytogenic compounds on growth performance and immune response of broilers. Journal of Applied Animal Research, 45(1), 603–608.

NRC. (1994) Nutrient requirements of poultry, 9th Revised edn. National Academy Press, Washington, DC.

ODEDIRE, J.A. and OLOIDI, F.F. (2011). Processing wild sunflower (Tithonia diversifolia) leaves as forage supplement in ruminant diet: effect of air-drying method on antinutritive components. SAADC 2011 strategies and challenges for sustainable animal agriculture-crop systems, Volume III: full papers. Proceedings of the 3rd International Conference on sustainable animal agriculture for developing countries, Nakhon Ratchasima, Thailand, 26–29 July, 2011.

ODUNSI, A.A., FARINU, G.O. and AKINOLA, J.O. (1996). Influence of dietary wild sunflower (Tithonia diversifolia Helms A.Gray) leaf meal on layers performance and egg quality. Nigerian Journal of Animal Production, 25, 28–32.

OLAYENI, T.B., FARINU, G.O., TOGUN, V.A., ADEDEJI, O.S. and ADERINOLA, A.O. (2006) Performance and haematological characteristics of weaner pigs fed wild sunflower (Tithonia diversifolia hemsl A Gray) leaf meal. Journal of Animal and Veterinary Advances, 5(6) 499–502.

OLORUNTOLA, O.D., AGBEDE, J.O., AYODELE, S.O. and OLORUNTOLA, D.A. (2018). Neem, pawpaw, and bamboo leaf meal dietary supplementation in broiler chickens: Effect on performance and health status. Journal of Food Biochemistry. e12723. https://doi.org/10.1111/jfbc.12723

OLORUNTOLA, O.D., AYODELE, S.O., ADEYEYE S.A, JIMOH, A.O., OLORUNTOLA, D.A. and OMONIYI, S.I. (2020). Pawpaw leaf and seed meals composite mix dietary supplementation: effects on broiler chicken’s performance, caecum microflora, and blood analysis. Agroforestry Systems, 94, 555–564. https://doi.org/10.1007/s10457-019-00424-1

OLUWASOLA, T.A. and DAIRO, F.A.S. (2016). Proximate composition, amino acid profile and some anti-nutrients of Tithonia diversifolia cut at two different times. African Journal of Agriculture and Research, 11(38), 3659–3663.

OMOKHUA, A.G., ABDALLA, M.A., VAN STADEN, J. and McGAW, L.J. (2018). A comprehensive study of the potential phytomedicinal use and toxicity of invasive Tithonia species in South Africa. BMC Complementary and Alternative Medicine, 18, 272. https://doi.org/10.1186/s12906-018-2336-0

ONYIMONYI, A.E., CHUKWUMA, P.C. and CHINENYE, I (2012). Growth and hypocholesterolemic properties of dry garlic powder (Allium sativum) on broilers. African Journal of Biotechnology, 11(11), 2666-2671.

OSUNTOKUN, O.T., JEMILAIYE, T.A., YUSUF-BABATUNDE, A.M. and AKELE, E.O. (2018). Antimicrobial properties, phytochemical composition, and phenotypic resistance pattern of selected enteropathogenic microorganism on Ageratum conyzoides (Goat weed) leaf extract. International Journal of Microbiology, 7(2),18–128.

PULIDO, R., BRAVO, L. and SAURA-CALIXTO, F. (2002). Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agriculture and Food Chemistry, 48, 3396–3402.

PUVACA, N., Lj. KOSTADINOVIC, LJ., POPOVIC, S., LEVIC, J., LJUBOJEVIC, D., TUFARELLI, V., JOVANOVIC, R., TASIC, T., IKONIC, P. and LUKAC, D (2015). Proximate composition, cholesterol concentration and lipid oxidation of meat from chickens fed dietary spice addition (Allium sativum, Piper nigrum, Capsicum annuum). Animal Production Science, 56(11), 1920–1927.

ROTRUCK, J.T., POPE, A.L., GANTHER, H.E., SWANSON, A.B., HAFEMAN, D.G. and HOEKSTRA, W.G. (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science, 179, 588–590.

SANTOS, R.F., NUNES,B.M., SA, R.D., SOARES, L.A.L. and RANDAU, K.P. (2016). Morpho-anatomical study of Ageratum conyzoides. Revista Brasileira de Farmacognosia, 26, 679–687. http://dx.doi.org/10.1016/j.bjp.2016.07.002

SHAILAJAN, S., WADKE, P., JOSHI, H. and TIWARI, B. (2013). Evaluation of quality and efficacy of an ethnomedicinal plant Ageratum conyzoides L. in the management of pediculosis. Journal of Young Pharmacists, 5(4), 139–143.

SHASTRY, G.A. (1983). Veterinary clinical pathology (2nd ed.). New Delhi, India: CBS Publishers and Distributors.

SHEN, M., XIE, Z., JIA, M., LI, A., HAN, H., WANG, T. and ZHANG, L. (2019). Effect of Bamboo leaf extract on antioxidant status and cholesterol metabolism in broiler chickens. Animals, 9, 699.

SOFOWORA, A. (1993). Medicinal Plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan.

TIJANI, L.A., AKANJI, A.M., AGBALAYA, K. and ONIGEMO, M. (2015). Haematological and serum biochemical profiles of broiler chickens fed diets containing moringa leaf meals. Journal of Tropical Agriculture, Food, Environment, and Extension, 14(3), 7–11.

TUNGMUNNITHUM, D., THONGBOONYOU, A., PHOLBOON, A. and YANGSABAI (2018) Flavonoids and other phenolic compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines (Basel), 5(3), 93.

VALENZUELA-GRIJALVA, N.V., PINELLI-SAAVEDRA, A., MUHLIA-ALMAZAN, A., DOMINGUEZ-DIAZ, D. and GONZALEZRIOS, H. (2017). Dietary inclusion effects of phytochemicals as growth promoters in animal production. Journal of Animal Science and Technology, 59, 8.  https://doi.org/10.1186/s40781-017-0133-9

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